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- W2361970093 abstract "A method of polymerase chain reaction(PCR) and capillary electrophoresis(CE) with optimization of response surface methodology has been developed for the detection of Norovirus in human diarrheal samples.The primers for PCR amplification were selected according to the highly conservative region of Norovirus.Response surface methodology was employed to determine the optimal experiment conditions in capillary electrophoresis.The PCR products were detected by using a laser-induced fluorescence detector and 0.5% MC sieving buffer with SYBR Gold under the optimal conditions within 9 minutes.At the same time,PCR products were sequenced and compared with the prototype sequences from Gene Bank.The uniformity was as high as 99%.The relative standard deviations of both interday and intraday migration time for the detected DNA fragments were 1.09%-1.30% and 1.77%-2.60%,respectively.Compared with agarose gel electrophoresis,the proposed method is rapid,sensitive,accurate and appropriate for the detection of Norovirus in diarrheal samples." @default.
- W2361970093 created "2016-06-24" @default.
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- W2361970093 date "2013-01-01" @default.
- W2361970093 modified "2023-09-26" @default.
- W2361970093 title "Rapid detection of norovirus in diarrheal samples by RT-PCR-capillary electrophoresis with optimization of response surface methodology" @default.
- W2361970093 hasPublicationYear "2013" @default.
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