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- W2361972017 abstract "Objective: To clone and analyze the HCV-1b gene fragments isolated by Restriction Display technique(RD) Methods:Restriction enzyme Sau3A Ⅰwas chosen to digest the full-length HCV-1b cDNAs. The products were classified and re-amplified by RD-PCR, the RD-PCR fragments were purified and cloned into the pMD18-T vectors. The recombinant plasmids were extracted from positive clones and the target gene fragments were sequenced. Results:The target HCV gene fragment ranging from 200 to 1000bp were isolated and sequenced which proved to be gene fragments derived from the HCV-1b genome. A total of 20 different cDNA fragments were quickly obtained. Conclusion:RD technique is of great value in obtaining a large number of size-comparable gene probes, which are suitable for the construction of cDNA fragments library and preparation of microarray probes." @default.
- W2361972017 created "2016-06-24" @default.
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- W2361972017 date "2004-01-01" @default.
- W2361972017 modified "2023-09-25" @default.
- W2361972017 title "Cloning and sequence analysis of HCV-1b gene fragments isolated by the RD method" @default.
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