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- W2361982011 abstract "Objective:To observe the effect of acupuncture on subunit p85 of phosphatidyl inositol kinase 3(PI3K) in skeletal muscle of insulin-resistance rats.Methods:Twenty-four SD rats were randomized into blank group,model group and acupuncture group.Rat models of insulin resistance were induced by feeding of high glucose,high fat and high salt diet.Glucose oxidase method,enzyme linked immunosorbent assay(ELISA),real-time fluorescent quantitative polymerase chain reaction(PCR),and western-blotting method were used for the detection of fasting plasma glucose(FPG) content,fasting plasma insulin(FINS) level,insulin sensitivity index,serum C-peptide(C-P) and PI3K p85 mRNA and protein expression in skeletal muscle of different groups respectively.Results:Compared with the blank group,FPG,FINS,C-P,PI3K p85α mRNA expression and PI3K p85 protein expression were increased,while ISI was decreased in the model group(P 0.01).FPG,FINS,C-P,PI3K p85α mRNA expression and PI3K p85 protein expression were decreased(P 0.05 or P 0.01),while ISI was increased in acupuncture group(P 0.01).Conclusion:Intervention of acupuncture can regulate the over-expression of PI3K p85 protein in skeletal muscle of insulin-resistance rats,enhance the activity of PI3K,which may be one of the therapeutic mechanisms of acupuncture for insulin resistance." @default.
- W2361982011 created "2016-06-24" @default.
- W2361982011 creator A5047277850 @default.
- W2361982011 date "2010-01-01" @default.
- W2361982011 modified "2023-09-28" @default.
- W2361982011 title "Effect of Acupuncture on PI3K p85 Expression in Skeletal Muscle of Insulin-resistance Rats" @default.
- W2361982011 hasPublicationYear "2010" @default.
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