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- W2361985979 abstract "The termination codon of human leukemia inhibitory factor gene was deleted by PCR mutation, in order to put the gene into the same reading frame together with the myc epitope and 6 × His tag in the expression vector pPICZαA. The constmcted expression vector pPICZαA-hLIFM was integrated into the genomic DNA of Pichia pas-toris X-33. SDS-PAGE and Western blot analysis.of the culture supernatants showed that hLIF gene were expressed as a 61 000 fused protein with the desired immunogenicity. Density scanning of the SDS- PAGE gel revealed that the targeted protein accounted for 33.3% of the total protein in the supernatants and reached highest level after 48hrs of methanol induction. The expressed products can inhibit the clone formation of murine' s teratoma cells F9." @default.
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- W2361985979 date "2001-01-01" @default.
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- W2361985979 title "Fusion Expression of Human Leukemia Inhibitory Factor Gene in Yeast" @default.
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