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- W2362019993 abstract "Objective To investigate the effect of alginate beads on mesenchymal stem cells (MSCs) when coculture and explore their possible application in bone tissue engineering. Methods Alginate beads were produced by dropping. The development of pH value and generational situation of MSCs in alginate beads solution at different concentrations was detected respectively by pH meter, MTT and flow cytometry. Results MSCs attached on alginate beads. The pH values of 0.02, 0.2 and 2.0 g/L alginate beads solution were fluctuated around 7.20, 7.20 and 6.70 respectively. The OD value detected by MTT method in above 3 groups was similar to blank cell control group. The analysis of cell cycle showed that the rates of S stage cells in alginate solution were equal to or larger than the control group on 3 rd day and these rates on 7 th day in alginate solution were similar to the control group. Conclusion The results suggest that alginate beads solution lower than 2.0 g/L has less effects on the growth of MSCs and reaches the microenvironment needs of MSCs." @default.
- W2362019993 created "2016-06-24" @default.
- W2362019993 creator A5071709424 @default.
- W2362019993 date "2007-01-01" @default.
- W2362019993 modified "2023-09-26" @default.
- W2362019993 title "Preparation of alginate beads and their effect on co-cultured MSCs" @default.
- W2362019993 hasPublicationYear "2007" @default.
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