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- W2362034543 abstract "AIM: To investigate the protective effect of emodin on hepatic cells after simulated cold ischemia-reperfusion injury.METHODS: Glucose-oxygen deprivation,low temperatrue and subsequent reoxygenation,rewarming were used to induce ischemia-reperfusion injury in cultured hepatic cells which were divided into 4 groups: control group,emodin treated groups(100,10,1 μmol/L groups).The contents of lactate dehydrogenase(LDH),malondialdehyde(MDA)and superoxide dismutase(SOD) were tested in cell supernatants.The reactive oxygen species(ROS)were determined by flow cytometry(FCM).RESULTS: In middle and high density emodin treated groups,LDH were(198.83±14.22)U/L and(179.67±18.57)U/L respectively,which were significantly lower than(351.33±34.16)U/L in control group(P0.01);MDA were(7.80±0.88)nmol/mL and(4.97±0.71)nmol/mL respectively,which were significantly lower than(16.67±1.14)nmol/mL in control group(P0.01);SOD were(14.32±1.53)U/mL and(18.64±1.38)U/mL respectively,which were significantly higher than(8.56±0.94)U/mL in control group(P0.01).In high,middle and low concentration emodin treated groups,ROS positive cell rate were 0.1140±0.0037,0.1599±0.0039 and 0.9527±0.0080,respectively,which were significantly lower than 0.9974±0.0006 in control group(P0.01).CONCLUSION: Emodin could effectively protect hepatic cells after simulated cold ischemia-reperfusion,and its protective effect is probably associated with reducing intracellular ROS level." @default.
- W2362034543 created "2016-06-24" @default.
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- W2362034543 date "2008-01-01" @default.
- W2362034543 modified "2023-09-23" @default.
- W2362034543 title "Protective effect of emodin against simulated cold ischemia-reperfusion injury in cultured hepatic cells and related mechanism" @default.
- W2362034543 hasPublicationYear "2008" @default.
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