Matches in SemOpenAlex for { <https://semopenalex.org/work/W2362043485> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2362043485 abstract "Objective:To study on establishment of human umbilical vein endothelial cells model of hypertensive disorder complicating pregnancy(HDCP),as well as to assay the angiotensin Ⅱ expression. Methods: Human umbilical vein endothelial cells were isolated by filling umbilical weins with digestive 1%Trypsin after PBS washed,then the cells were cultured in McCoy's 5Ac cell media;The morphology and source of isolated cells were assayed by acridine orange staining and immunohistochemistry technique;the differential expressions of Ang Ⅱ in hUVECs between HDCP and WT were examined by western blotting. Results: Large numbers of primary cells can be get with 1% Trypsin digestive,which can be cultured into 6th passage in McCoy's 5Ac cell media without any growht factors. The AO staining indicated that the cells had slippery verges and inerratic nucleolus. The results of immunohistochemistry indicated that hVⅡI and hVEGF were expressed highly in the isolated cells.The expression level of Ang Ⅱ in HDCP hUVECs (90.20 ± 5.84)%,P= 0.0130)was much high than WT's. Conclusion: Perfusion with 1%Trypsin and cultured with McCoy's 5Ac cell media is an effective method for estalishing human umbilical vein endothelial cells of hypertensive disorder complicating pregnancy,and in which the expression level of Ang Ⅱ is high." @default.
- W2362043485 created "2016-06-24" @default.
- W2362043485 creator A5074920934 @default.
- W2362043485 date "2009-01-01" @default.
- W2362043485 modified "2023-09-25" @default.
- W2362043485 title "Study on Establishment of Human Umbilical Vein Endothelial Cells of Hypertensive Disorder Complicating Pregnancy and Angiotensin II Expression" @default.
- W2362043485 hasPublicationYear "2009" @default.
- W2362043485 type Work @default.
- W2362043485 sameAs 2362043485 @default.
- W2362043485 citedByCount "0" @default.
- W2362043485 crossrefType "journal-article" @default.
- W2362043485 hasAuthorship W2362043485A5074920934 @default.
- W2362043485 hasConcept C126322002 @default.
- W2362043485 hasConcept C134018914 @default.
- W2362043485 hasConcept C142724271 @default.
- W2362043485 hasConcept C153911025 @default.
- W2362043485 hasConcept C16685009 @default.
- W2362043485 hasConcept C172680121 @default.
- W2362043485 hasConcept C202751555 @default.
- W2362043485 hasConcept C204232928 @default.
- W2362043485 hasConcept C2777218350 @default.
- W2362043485 hasConcept C2777411675 @default.
- W2362043485 hasConcept C2778194526 @default.
- W2362043485 hasConcept C2779234561 @default.
- W2362043485 hasConcept C2910785818 @default.
- W2362043485 hasConcept C54355233 @default.
- W2362043485 hasConcept C55493867 @default.
- W2362043485 hasConcept C71924100 @default.
- W2362043485 hasConcept C74864618 @default.
- W2362043485 hasConcept C86803240 @default.
- W2362043485 hasConceptScore W2362043485C126322002 @default.
- W2362043485 hasConceptScore W2362043485C134018914 @default.
- W2362043485 hasConceptScore W2362043485C142724271 @default.
- W2362043485 hasConceptScore W2362043485C153911025 @default.
- W2362043485 hasConceptScore W2362043485C16685009 @default.
- W2362043485 hasConceptScore W2362043485C172680121 @default.
- W2362043485 hasConceptScore W2362043485C202751555 @default.
- W2362043485 hasConceptScore W2362043485C204232928 @default.
- W2362043485 hasConceptScore W2362043485C2777218350 @default.
- W2362043485 hasConceptScore W2362043485C2777411675 @default.
- W2362043485 hasConceptScore W2362043485C2778194526 @default.
- W2362043485 hasConceptScore W2362043485C2779234561 @default.
- W2362043485 hasConceptScore W2362043485C2910785818 @default.
- W2362043485 hasConceptScore W2362043485C54355233 @default.
- W2362043485 hasConceptScore W2362043485C55493867 @default.
- W2362043485 hasConceptScore W2362043485C71924100 @default.
- W2362043485 hasConceptScore W2362043485C74864618 @default.
- W2362043485 hasConceptScore W2362043485C86803240 @default.
- W2362043485 hasLocation W23620434851 @default.
- W2362043485 hasOpenAccess W2362043485 @default.
- W2362043485 hasPrimaryLocation W23620434851 @default.
- W2362043485 hasRelatedWork W2017981137 @default.
- W2362043485 hasRelatedWork W2019922358 @default.
- W2362043485 hasRelatedWork W2165694350 @default.
- W2362043485 hasRelatedWork W2347688657 @default.
- W2362043485 hasRelatedWork W2353915214 @default.
- W2362043485 hasRelatedWork W2355003578 @default.
- W2362043485 hasRelatedWork W2355782184 @default.
- W2362043485 hasRelatedWork W2356035386 @default.
- W2362043485 hasRelatedWork W2361697175 @default.
- W2362043485 hasRelatedWork W2362421842 @default.
- W2362043485 hasRelatedWork W2365959849 @default.
- W2362043485 hasRelatedWork W2367241302 @default.
- W2362043485 hasRelatedWork W2369611500 @default.
- W2362043485 hasRelatedWork W2371337202 @default.
- W2362043485 hasRelatedWork W2372928802 @default.
- W2362043485 hasRelatedWork W2374174892 @default.
- W2362043485 hasRelatedWork W2388449367 @default.
- W2362043485 hasRelatedWork W2389978477 @default.
- W2362043485 hasRelatedWork W2393289682 @default.
- W2362043485 hasRelatedWork W2393860336 @default.
- W2362043485 isParatext "false" @default.
- W2362043485 isRetracted "false" @default.
- W2362043485 magId "2362043485" @default.
- W2362043485 workType "article" @default.