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- W2362044853 abstract "Objective:To screen the effective parts of Rhizoma Bletillae extracts against mice melanoma B16 cells and evaluate its apoptosis-inducing effect.Methods:Rhizoma Bletillae was extracted by reflux extracting methods with 95% ethanol,then sub-fractionated into petroleum benzine part,chloroform part,acetoacetate part,n-butyl alcohol and water part by system solvent extraction method.The antitumor activity against mice melanoma B16 cells was detected by CCK-8 method;additionally,the apoptosis-inducing effect was evaluated by fluorescent microscopy method and flow cytometric method.Results:The chloroform part and acetoacetate part of the extracts of Rhizoma Bletillae were the active subfractions,which could inhibit B16 cell growth in a concentration-dependent manner,and the former with the strongest activity with IC50 at 60.99 g/mL.Fluorescent microscopy and flow cytometric methods indicated the chloroform part could effectively induce B16 cells apoptosis.Conclusion:Rhizoma Bletillae with antitumor activity against B16 cells,the chloroform part is the active subfraction,and inducing apoptosis was one of the mechanism,which is worthy of further exploitation." @default.
- W2362044853 created "2016-06-24" @default.
- W2362044853 creator A5085142395 @default.
- W2362044853 date "2013-01-01" @default.
- W2362044853 modified "2023-09-26" @default.
- W2362044853 title "Study of Rhizoma Bletillae Striatae Extracts in Inducing Apoptosis on Mice Melanoma B16 Cells" @default.
- W2362044853 hasPublicationYear "2013" @default.
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