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- W2362096243 abstract "Objective A MTT method for evaluate the efficacy of drugs for anti HIV 1 infection in vitro,was developed with reference materials.Methods Briefly,Anti HIV assay was first in progress with 96 well plastic microtiter trays.Four days after infection,20μl/well of MTT(0.7mg/ml) was added,the trays were incubated at 37℃ in a CO 2 incubator for 4h.150μl/well of medium was removed,then 100μl/well of DMSO was added.The absorbance (OD540) were read,then the percent protection of drugs in HIV infected cells was calculated.Results CC50=0.3354mg/ml,EC50=0.0864mg/ml of HL,MTT assay is more sensitive then CPE assay.Conclusion By the MTT assay,a rapid,objective,accurate assay results can achieve with absorbance,therefore,enhance quality and efficiency of the experiments." @default.
- W2362096243 created "2016-06-24" @default.
- W2362096243 creator A5052670174 @default.
- W2362096243 date "1998-01-01" @default.
- W2362096243 modified "2023-09-23" @default.
- W2362096243 title "Apply a MTT assay to studying on anti HIV drugs" @default.
- W2362096243 hasPublicationYear "1998" @default.
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