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- W2362110004 abstract "Objective To analyze the biological features of Coxsachie virus A16(CVA16)which caused Hand-foot-mouth Disease(HFMD)epidemics in Liaocheng area,master its gene characteristics,so as to provide the etiological data for prevention and control of HFMD. Methods Specimens from HFMD cases were collected in Liaocheng area during 2010-2013 and detected for CVA16 virus by Real-time PCR.Positive specimens were separated by using human rhabdomyosarcoma(RD)cells for virus isolation.The virus isolates were amplified and identified using RT-PCR for VP1 gene amplification.The amplified products were sent to be sequenced and the homology analysis and the construction of phylogenetic trees of CVA16 isolates were carried on by DNAStar and MEGA 4.0. Results A total of 862 specimens were collected and tested for enterovirus RNA during 2010-2013 in Liaocheng area,and 639 specimens were positive for universal primers for enterovirus.181 specimens were CVA16 positive,and of which 109 virus isolates were separated.According to the gene analysis,genotype B1 of CVA16was prevalent during 2010-2013.The gene types of B1 aand B1bwere both circulated and prevalent in 2010 and 2011,B1 bwas prevalent during 2012-2013.The homology analysis on nucleotide and amino acid for VP1 region of CVA16 virus isolates from Liaocheng area illustrated that the gene homologies were respectively 91.0%-97.0% and 98.3%-100%.Compared with the proximal subtype of representative strains,the variation on amino acid for VP1 region of CVA16 virus isolates from Liaocheng area usually occurred in the VP1 23rd(L→M). Conclusion B1 genotype is dominant among CVA16 isolates during 2010- 2013 in Liaocheng area including B1 aand B1btwo sub- types.Homology analysis indicates that partial B1 asubtypes have a close relationship with isolates from Taiwan province in China and neighboring countries.B1 bsubtypes have a close relationship with isolates from northern China and constitute a same group in the phylogenetic tree.There are different transmissions of HFMD in Liaocheng area.CVA16 isolates are relatively conservative,but there has been a certain degree of variation." @default.
- W2362110004 created "2016-06-24" @default.
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- W2362110004 date "2015-01-01" @default.
- W2362110004 modified "2023-09-23" @default.
- W2362110004 title "Analysis on gene characteristics of VP1 regions of Coxsachie virus A16 in enterovirus A group and the epidemiological characteristics of hand-foot-mouth disease,Liaocheng area,2010-2013" @default.
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