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- W2362128886 abstract "A two-dimensional electrophoresis( 2-DE) system was established and optimized to study the proteome of Eucalyptus leaves. The results showed that TCA / acetone / phenol extraction was the most suitable extraction method for eucalyptus leaves. When the lysis buffer was consisted of 7 mol·L- 1urea,2 mol·L- 1thiourea,0. 04 g·m L- 1CHAPS,60 mmol·L- 1DTT and 0. 5%( volume fraction) IPG buffer,the protein sample dissolved quite well. It was applicable to dissolving eucalyptus leaf protein. A higher-quality 2-DE map could be got using a 18 cm,p H 4- 7 linear strip with 50 μg sample volume by silver staining method." @default.
- W2362128886 created "2016-06-24" @default.
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- W2362128886 date "2015-01-01" @default.
- W2362128886 modified "2023-09-23" @default.
- W2362128886 title "Establishment and optimization of 2-DE technique system in leaf proteome of Eucalyptus" @default.
- W2362128886 hasPublicationYear "2015" @default.
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