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- W2362149382 abstract "Objective To study the influence of NS1A protein of avian influenza virus on the proliferation of human lung cancer SPC-A1 cells.Methods Transfect human lung cancer SPC-A1 cells with recombinant plasmid pVAX1-NS1A in mediation of liposome.Determine the inhibiting effect on cell proliferation by MTT method,the apoptosis rate by Annexin V FITC kit,PI and flow cytometry,and the cell cycle by PI and flow cytometry.Identify the expressed product in transfected SPC-A1 cells by Western blot.Results NS1A protein showed a time-dependent inhibiting effect on the proliferation of SPC-A1 cells.The apoptosis rate of SPC-A1 cells transfected with recombinant plasmid pVAX1-NS1A reached 38.17%,and the percentage of cells at G0/G1 stages increased as time goes on.The cycle of transfected SPC-A1 cells was blocked at stage G1.Wesetent blot showed a specific reaction band with relative molecular mass of about 30 000,which was consistent with that of NS1A protein.Conclusion The NS1A protein of avian influenza virus inhibited the proliferation and induced the apoptosis of human lung cancer SPC-A1 cells." @default.
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- W2362149382 date "2007-01-01" @default.
- W2362149382 modified "2023-09-24" @default.
- W2362149382 title "Induction of Apoptosis of Human Lung Cancer SPC-A1 Cells with NS1A Protein of Avian Influenza Virus" @default.
- W2362149382 hasPublicationYear "2007" @default.
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