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- W2362155393 abstract "Objective: To construct the RPAP3(TPR domain)-containing lentivirus,and investigate the influence of the overexpression of PRAP3 on cell cycle.Methods: RPAP3 with other homology proteins were compared by using sequence analysis tools.Then RPAP3's nuclear localization signal sequence was analyzed,and produced transient expressing plasmid and its subcellular localization was studied by laser scanning confocal microscope.The Yeast two-hybrid and GST-Pulldown we used To invest the coaction of RPAP3 with HSP70 and the reaction site,.localization by laser scanning confocal microscope.To invest the coaction of RPAP3 with HSP70 and the reaction site,we used the Yeast two-hybrid and GST-Pulldown.A lentivirus expression vector wAS constructed,and infected MCF7 cells.Flow cytometry was used to to investigate the effect on cell cycle.Results: RPAP3 exists among many species and was highly conserved.It has a typical nuclear localization single(RKKKKGKAKESSKKTRE) and is mainly distributed in the cell nucleus under laser scanning confocal microscope.The interaction was confirmed,which happens between the three-TPR domain and hsp70 terminal GPTIEEVD by Yeast two-hybrid and GST-Pulldown.The results of flow cytometry analysis showed that the overexpression of RPAP3 inhibit the cell cycle and increased apoptosis.Conclusions: RPAP3 can interact with hsp70 and hsp90 and it happens between the three-TPR domain and hsp70 terminal GPTIEEVD.Overexpression of RPAP3 influenced the cell cycle and apoptosis." @default.
- W2362155393 created "2016-06-24" @default.
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- W2362155393 date "2012-01-01" @default.
- W2362155393 modified "2023-09-22" @default.
- W2362155393 title "Functional Study of TPR Domain in RPAP3" @default.
- W2362155393 hasPublicationYear "2012" @default.
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