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- W2362156594 abstract "To elucidate the expression and regulation of interleukin-18 (IL-18) in human leukemia cell line J6-1, and investigate its possible role in leukemogenesis.IL-18 mRNA expression was detected using reverse transcriptase-ploymerase chain reaction (RT-PCR), and the specificity of IL-18 cDNA was verified by restriction endonucleases analysis. To sequence and analyze its cDNA alignment, a recombinant plasmid of IL-18 was constructed. Employing the antisense technique, we ionvestigated the effects of IL-18 antisense oligodeoxynucleiotide (ASODN) on the proliferation of J6-1 cells.J6-1 cells constitutively expresses IL-18 mRNA, and its cDNA sequence shared 99% homology with the published IL-18 sequence,except that a synonymous point mutation (Codon35 UCA to UCC) was confirmed by sequencing and alignment analysis. Peripheral blood mononuclear cells (PBMC) of healthy volunteers weakly expresses IL-18 mRNA (0.13 +/- 0.05), while CpG-ODN significantly up-modulated IL-18 expression in PBMC (0.82 +/- 0.24), but not in J6-1 cells. Compared with K562, HL-60, U937 and LCL cells, IL-18 mRNA level expression of in J6-1 cells was the highest (0.98 +/- 0.29). ASODN of IL-18 markedly inhibited the proliferation of J6-1 cells (inhibition rate 43.3%), but did not show significant effects on HL-60 cells (inhibition rate 16.7%).J6-1 cells constitutively expresses IL-18 mRNA, and no significant mutation was detected coding region of IL-18 cDNA. IL-18 might be a positive regulation factor for the proliferation of J6-1 cells after in an autocrine model." @default.
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- W2362156594 date "2001-05-25" @default.
- W2362156594 modified "2023-09-23" @default.
- W2362156594 title "[Expression of interleukin-18 in human leukemia cell line J6-1 and its significance]." @default.
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