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- W2362169010 abstract "To prepare monoclonal antibody(mAb) against serum immunoglobulin(Ig) of tilapia and characterize its immunological feature,female Balb/c mice were immunized with tilapia Ig purified by Protein A affinity chromatography.Indirect ELISA and Western blot were employed to screen the hybridoma cells by determining the specificities and titer of the produced mAbs.SDS-PAGE analysis showed that molecular weight of tilapia Ig heavy chain and light chain was 77 kD and 27 kD respectively.Four hybridoma cell strains that could stably secret mAb against the tilapia Ig were obtained,and named as 3D9,9D4,8G3 and 7B7,which all belongs to IgG1.Titer of the four mAbs was ranged from 1∶3 200 to 1∶6 400 tested by ELISA.3D9 was able to recognize the heavy chain of tilapia Ig,while the other three mAbs could bind to light chain of Ig.Further experiments proved that all of them only bound to Ig of tilapia Oreochromis niloticu specifically,and have not any cross reaction with the Ig of Cienoyharyngodoni dellus,Carrassius auratus,Ietalurus Punetaus,Silurus meridionalis Chen or Lateolabrax japomcus.Using mAbs,tilapia serum antibodies against Aeromonas hydrophila could be detected by ELISA at day 8 after the first immunization,and the peak of titer was appeared at the third week after the third immunization.All of these results demonstrated that the prepared mAbs were highly specific to the Ig of tilapia,and had great potential to be used for analyzing the structure of tilapia Ig,monitoring the immunity level and pathogen diagnosis." @default.
- W2362169010 created "2016-06-24" @default.
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- W2362169010 date "2013-01-01" @default.
- W2362169010 modified "2023-09-24" @default.
- W2362169010 title "Preparation and characterization of monoclonal antibodies against serum immunoglobulin from Oreochromis niloticus" @default.
- W2362169010 hasPublicationYear "2013" @default.
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