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- W2362184482 abstract "Objective To study the post-thaw survival rate and differentiation ability of human bone marrow mesenchymal stem cells(BMSCs). Methods Human BMSCs were isolated and purified in vitro,its purity was detected by flow cytometer.The cells were offered cryopreservation by using vitrification frozen.The survival rate was measured after thawing.BMSCs were then cultured in DMEM/F12 supplemented with 10 μg/L bFGF and 10% fetal bovine serum pre-induce for two days,and then induced by RA(0.1 μmol/L) and 10 μg/L Glial cell line derived neurotrophic factor(GDNF) for formal induction for three days.the expressions of Nestin,MAP2,MBP,GFAP were detected by immunofluorescence staining. Results Strong expressions of CD44 and CD90 were noted in BMSCs,but no CD34 and CD45;post-thaw cell survival was 95%.After induction,the expressions of BMSCSMBP and GFAP were strongly positive,and that of Nestin and MAP2 weakly positive. Conclusion The survival rate of post-thaw BMSCs is high and can be successfully induced to become neuroglial cells." @default.
- W2362184482 created "2016-06-24" @default.
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- W2362184482 date "2013-01-01" @default.
- W2362184482 modified "2023-09-24" @default.
- W2362184482 title "BIOLOGICAL ACTIVITY OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS AFTER VITRIFICATION FROZEN AND THAW" @default.
- W2362184482 hasPublicationYear "2013" @default.
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