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- W2362208665 abstract "Objective To obtain articular chondrocyte from patients with osteoarthritis by primary culture,to explore the relationship between different concentrate[1,25-(OH)2D3]and proliferation,apoptosis of chondrocytes.Methods Human articular chondrocytes were obtained by modified digestive method with enzyme in vitro and indentified by alkaline phosphatase(AKP) staining.OA Chondrocytes were cultured in medium with different concentrate[1,25-(OH)2D3].MMT method was used to assay proliferation of chondrocytes.Optical density(OD) at 490nm was determined by ELISA.Flow cytometry was used to assay the apoptosis ratio at different time point.Results 10-5 umol/L[1,25-(OH)2D3]can promote the proliferation of chondrocytes obviously,and the proper react time point is 48 hour(P0.01).At the same time 10-5 umol/L[1,25-(OH)2D3]can inhibit the apoptosis ratio obviously at 48 hour time point.Conclusion To promote the proliferation and inhibit the apoptosis of OA chondrocytes in vitro,the better concentration of[1,25-(OH)2D3]is 1×10-5 umol/L.48 hour after[1,25-(OH)2D3]added is the best time for the experiment objective." @default.
- W2362208665 created "2016-06-24" @default.
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- W2362208665 date "2012-01-01" @default.
- W2362208665 modified "2023-09-24" @default.
- W2362208665 title "The Effects of Vitamin D3 on Proliferation and Apoptosis of Primary Cultured Chondrocytes from Human Articular Cartilage" @default.
- W2362208665 hasPublicationYear "2012" @default.
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