Matches in SemOpenAlex for { <https://semopenalex.org/work/W2362232895> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2362232895 abstract "Objective To clone and express the tissue inhibitor of metalloproteinase homologue(TIMP like,TIMPL),Ad-TIMPL-1,from the hookworm Ancylostoma duodenale.Methods The 5' and 3' end of cDNA encoding Ad-TIMPL-1 were amplified by 3'RACE and RT-PCR from the cDNA of adult A.duodenale,respectively.The nucleotide sequence encoding mature Ad-TIMPL-1 was ligated into pET32a to construct the recombinant plasmid pET32a/Ad-TIMPL-1.The recombinant plasmid was transformed into E.coli BL21(DE3),and then expressed by inducing with IPTG and analysed by SDS-PAGE.Results The full-length cDNA of Ad-TIMPL-1 was obtained from A.duodenale and deposited in GenBank(accession no.EF495071).The full-length cDNA contains an open reading frame of 396 bp,which encoding the Ad-TIMPL-1 precursor of 132 amino acids with a signal peptide of 16 amino-acid-residue.The mature Ad-TIMPL-1 was expressed in E.coli BL21(DE3) after inducing by IPTG.Conclusion This study is the first report on the isolation,expression of a TIMP homologue from the human hookworm A.duodenale.Our results laid a foundation for futher research the biological function of Ad-TIMPL-1." @default.
- W2362232895 created "2016-06-24" @default.
- W2362232895 creator A5039886228 @default.
- W2362232895 date "2011-01-01" @default.
- W2362232895 modified "2023-09-28" @default.
- W2362232895 title "Cloning and prokaryotic expression of Ad-TIMPL-1,a tissue inhibitor of metalloproteinase homologue from Ancylostoma duodenale" @default.
- W2362232895 hasPublicationYear "2011" @default.
- W2362232895 type Work @default.
- W2362232895 sameAs 2362232895 @default.
- W2362232895 citedByCount "0" @default.
- W2362232895 crossrefType "journal-article" @default.
- W2362232895 hasAuthorship W2362232895A5039886228 @default.
- W2362232895 hasConcept C104317684 @default.
- W2362232895 hasConcept C153911025 @default.
- W2362232895 hasConcept C165901193 @default.
- W2362232895 hasConcept C167625842 @default.
- W2362232895 hasConcept C187882448 @default.
- W2362232895 hasConcept C19924922 @default.
- W2362232895 hasConcept C2777047024 @default.
- W2362232895 hasConcept C2777364306 @default.
- W2362232895 hasConcept C2777683361 @default.
- W2362232895 hasConcept C2779192670 @default.
- W2362232895 hasConcept C47289529 @default.
- W2362232895 hasConcept C50724178 @default.
- W2362232895 hasConcept C54355233 @default.
- W2362232895 hasConcept C61716771 @default.
- W2362232895 hasConcept C79029880 @default.
- W2362232895 hasConcept C86803240 @default.
- W2362232895 hasConcept C89423630 @default.
- W2362232895 hasConcept C90856448 @default.
- W2362232895 hasConceptScore W2362232895C104317684 @default.
- W2362232895 hasConceptScore W2362232895C153911025 @default.
- W2362232895 hasConceptScore W2362232895C165901193 @default.
- W2362232895 hasConceptScore W2362232895C167625842 @default.
- W2362232895 hasConceptScore W2362232895C187882448 @default.
- W2362232895 hasConceptScore W2362232895C19924922 @default.
- W2362232895 hasConceptScore W2362232895C2777047024 @default.
- W2362232895 hasConceptScore W2362232895C2777364306 @default.
- W2362232895 hasConceptScore W2362232895C2777683361 @default.
- W2362232895 hasConceptScore W2362232895C2779192670 @default.
- W2362232895 hasConceptScore W2362232895C47289529 @default.
- W2362232895 hasConceptScore W2362232895C50724178 @default.
- W2362232895 hasConceptScore W2362232895C54355233 @default.
- W2362232895 hasConceptScore W2362232895C61716771 @default.
- W2362232895 hasConceptScore W2362232895C79029880 @default.
- W2362232895 hasConceptScore W2362232895C86803240 @default.
- W2362232895 hasConceptScore W2362232895C89423630 @default.
- W2362232895 hasConceptScore W2362232895C90856448 @default.
- W2362232895 hasLocation W23622328951 @default.
- W2362232895 hasOpenAccess W2362232895 @default.
- W2362232895 hasPrimaryLocation W23622328951 @default.
- W2362232895 hasRelatedWork W153987944 @default.
- W2362232895 hasRelatedWork W2343100768 @default.
- W2362232895 hasRelatedWork W2345977400 @default.
- W2362232895 hasRelatedWork W2351398816 @default.
- W2362232895 hasRelatedWork W2351898451 @default.
- W2362232895 hasRelatedWork W2353777675 @default.
- W2362232895 hasRelatedWork W2355641250 @default.
- W2362232895 hasRelatedWork W2356544535 @default.
- W2362232895 hasRelatedWork W2359273176 @default.
- W2362232895 hasRelatedWork W2365991933 @default.
- W2362232895 hasRelatedWork W2366473978 @default.
- W2362232895 hasRelatedWork W2376383659 @default.
- W2362232895 hasRelatedWork W2382768397 @default.
- W2362232895 hasRelatedWork W2385223256 @default.
- W2362232895 hasRelatedWork W2386166069 @default.
- W2362232895 hasRelatedWork W2388382560 @default.
- W2362232895 hasRelatedWork W2388927158 @default.
- W2362232895 hasRelatedWork W2389626044 @default.
- W2362232895 hasRelatedWork W2390909326 @default.
- W2362232895 hasRelatedWork W978151442 @default.
- W2362232895 isParatext "false" @default.
- W2362232895 isRetracted "false" @default.
- W2362232895 magId "2362232895" @default.
- W2362232895 workType "article" @default.