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- W2362238002 abstract "Objective Microarray was used to study the toxic effect of Sudan I on the gene expression profile of human lymphocytes, with emphasis on the gene expression and tumorigenesis.Methods Peripheral blood lymphocytes were treated with 10 -5 mol/L Sudan I for 8 h. The total RNA from cells of the subjects of the treated group and the control group were isolated, then they were reversely transcribed and labeled with fluorescence dyes, and finally hybridized with human whole genome microarray (about 35,000 spots). Microarray data was extracted after microarray scanning, and the genes correlating to tumor were screened out and analyzed by bioinformatics.Results Among 306 up-regulated genes, 11 genes were found to be related to tumor formation. 181 genes were down-regulated, of which 16 genes were related with tumor development.Conclusion Sudan I could affect gene expression of lymphocytes, and reduced gene expression is an indicator of tumor development." @default.
- W2362238002 created "2016-06-24" @default.
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- W2362238002 date "2006-01-01" @default.
- W2362238002 modified "2023-09-23" @default.
- W2362238002 title "Gene Expression Profile of Sudan I-Treated Peripheral Blood Lymphocytes" @default.
- W2362238002 hasPublicationYear "2006" @default.
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