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- W2362294817 abstract "OBJECTIVE:To study the cytolytic mechanism of NK cell expanded in stem growth medium SCGM and stimulated with HSP70 peptide TKD against HSP70-positived pancreatic carcinomacells.METHODS:CD3-CD56+NK cells were obtained from human peripheral blood mononuclear(PBMC)in stem cell growth medium SCGM.The amount of IFN-γ produced by NK cells activated with HSP70 peptide TKD was quantified by ELISA technique.The expression of Granzyme B was quantified by ELISPOT technique.The cytolytic activity of TKD-activated NK cells against human pancreatic carcinoma sublines with and without HSP70 expression on their cell surface was analyzed by MTT assay.The relation between cytolytic activity and production of IFN-γ and Granzyme B produced by NK cells was analyzed.RESULTS:When NK cells were expanded from PBMC and stimulated with 2.0 μg/mL HSP70 peptide TKD,the amount of IFN-γ produced by NK TKD-activated cells increased up to(30.2±1.3) ng/mL compared to(8.9±0.7) ng/mL produced by unactivated NK cells(P0.05).There was significant statistical difference between them.And the expression of Granzyme B increased up to(21.2±0.6)% compared to(3.2±0.3)% expressed by unactivated NK cells,there was significant statistically different between them(P0.05).The cytolytic activity of TKD-activated NK cells against HSP70-negative pancreatic carcinoma sublines colo-was low and there was not statistical difference to unactivated NK cells.The cytolytic activity against HSP70 membrane-positive pancreatic carcinoma sublines colo+ was high and there was significantly statistical difference between TKD-activated NK cells and unactivated NK cells.The cytolytic activity was vated NK cells.CONCLUSIONS:The production of IFN-γ,the expression of Granzyme B and The cytolytic activity HSP70 membrane-positive pancreatic carcinoma sublines colo + is the highest when NK cells are stimulated with 2.0 μg/mL HSP70 peptide TKD.Our results indicate that the cytolytic activity against HSP70-positive tumor cells by NK cells is associated with the production of IFN-γ and Granzyme B by NK cells." @default.
- W2362294817 created "2016-06-24" @default.
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- W2362294817 date "2013-01-01" @default.
- W2362294817 modified "2023-09-23" @default.
- W2362294817 title "Cytolytic activity of NK cell stimulated with HSP70 peptide TKD against pancreatic carcinoma cell" @default.
- W2362294817 hasPublicationYear "2013" @default.
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