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- W2362300476 abstract "Aim:To study the effect of low oxygen tension on culturing human umbilical vein endothelial cells (HUVEC).Methods:HUVEC was cultured at low oxygen tension(3.94kPa = 29.58 mmHg)for 6 hours,then the lac- tate dehydrogenase(LDH),6-keto-PGF_(1α)in the medium and F-actin in the cells were measured at different time points. Results:The activity of LDH which was cultured in the solution at low oxygen tension for one hour was higher than that of the control,meanwhile,vacuolar degeneration around the nuclei of HUVEC was observed.Two hours after culture, the content of F-actin in cytoplasm of HUVEC was obviously decreased,6-keto-PGF_(1α)in the solution was incressed,and the cells contracted,separated and fell off.Conclusion:When oxygen pressure was below 3.94 kPa for 2 hours,the damage to HUVEC was irreversible." @default.
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- W2362300476 date "1996-01-01" @default.
- W2362300476 modified "2023-09-27" @default.
- W2362300476 title "Effect of Low Oxygen Tension on Culturing Human Umbilical Vein Endothelial Cells" @default.
- W2362300476 hasPublicationYear "1996" @default.
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