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- W2362311282 abstract "Objective:To observe invasion inhibiting effect of Hpa small-interfering RNA(siRNA) combined with arsenous acid on pancreatic cancer cells,so as to provide the theoretical foundation for further clinical use in the cancer treatment.Methods:Target gene(Hpa1-siRNA,Hpa2-siRNA,Hpa3-siRNA) fragments were diluted,annealed and connected with linear plasmid expression vector pGenesil-1.1,then pGenesil1.1/Hpa1-siRNA,pGenesil1.1/Hpa2-siRNA and pGenesil1.1/Hpa3-siRNA were reconstructed.The Hpa-siRNA was transfected into SW1990 pancreatic cancer cell by DOTAP Liposomes meanwhile the empty control and negative control group were designed.Those target genes were respectively transfected into Hpa-mRNA and Hpa protein expression was detected by RT-PCR and Western blot respectively.Transwell cab model was employed to test the ability of SW1990 cell.Results:Expression of Hpa-mRNA and Hpa-protein in SW1990 were obviously suppressed by Hpa1-siRNA,Hpa2-siRNA,and Hpa3-siRNA,and efficacy in Hpa2-siRNA better than in Hpa1-siRNA and Hpa3-siRNA(P0.05).With the improvement of arsenous acid concentration from 0.5,1.0,to 2.0mg/mL,the invasive inhibitory rates in pancreatic cancer increased from 7.78%,46.8%,to 76.8%,respectively,the differences had statistics significance(χ2=71.633,P0.001).Invasive ability of pancreatic cancer cells were completely inhabited by Hpa2-siRNA combined with arsenous acid(invasive inhibitory rates reached 100%).Conclusion:Hpa-siRNA and arsenous acid have co-inhibition effect on pancreatic cancer invasion." @default.
- W2362311282 created "2016-06-24" @default.
- W2362311282 creator A5063042333 @default.
- W2362311282 date "2011-01-01" @default.
- W2362311282 modified "2023-09-25" @default.
- W2362311282 title "Research on Heparanase siRNA Combined with Arsenous Acid Inhibiting Invasion of Pancreatic Cancer Cells in Vitro" @default.
- W2362311282 hasPublicationYear "2011" @default.
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