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- W2362321920 abstract "Objective To investigate the effects of tumor-suppressing gene,wild type PTEN gene mediated by adenovirus vector on proliferation,apoptosis and cell cycle of human myeloid leukemia primary cells and K562 cells lines in vitro.Methods The recombinated Ad-PTEN containing green fluorescent protein(GFP)was transfected into leukemia cells and K562 cell lines.The growth of leukemia cells was detected by MTT assay,and the apoptosis rate and cell cycle were assessed by flow cytometry.Besides,cell morphology was observed by light microscopy and electron microscopy.Cell proliferation and cell apoptosis were detected by TUNEL method.Cell colonies were cultured and the number of colonies were compared among groups.PTEN mRNA levels were examined by real-time fluorescent quantitative reverse transcriptional PCR(FQ-PCR),and PTEN protein was detected by Western blotting.Results The growth of K562 cell line was markedly suppressed after 200 multiplicity of infection(MOI)of Ad-PTEN-GFP was transfected.The maximal growth inhibition ratio was 35.2% and the maximal apoptosis rate was 30.0%.Cell cycle was arrested in G0/G1 phase.The percentage of G0/G1 phase cells increased [(54.9±2.51)% vs(78.5±4.13)%],while G2/M phase cells decreased [(30.2±1.91)% vs(13.6±1.02)%](P0.05).Conclusion The overexpression of PTEN gene can inhibit leukemia cell proliferation,promote cell apoptosis and results in cell cycle arrest at G0/G1 phase." @default.
- W2362321920 created "2016-06-24" @default.
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- W2362321920 date "2009-01-01" @default.
- W2362321920 modified "2023-09-26" @default.
- W2362321920 title "Effects of wild type PTEN gene on proliferation,apoptosis and cell cycle of leukemia cells" @default.
- W2362321920 hasPublicationYear "2009" @default.
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