Matches in SemOpenAlex for { <https://semopenalex.org/work/W2362340358> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2362340358 endingPage "459" @default.
- W2362340358 startingPage "455" @default.
- W2362340358 abstract "To study the structure,function and immunogenicity of the GP3 protein of highly pathogenic porcine reproductive and respiratory syndrome virus(PRRSV),the ORF3 gene of PRRSV was amplified by RT-PCR from PRRSV HuN4 strains and cloned into the baculovirus donor vector pFastBac HTb.The recombinant plasmid pF-ORF3 DNA was transformed into E.coli competent cells DH10Bac and the bacmid rBac-ORF3 was transfected into Sf9 insect cells.The recombinant baculovirus rAc-ORF3 was obtained and confirmed by PCR.Western blot and IFA confirmed that the GP3 was expressed in Sf9 cells.The expressed GP3 was purified and inoculated to BALB/c mice to prepare anti-GP3 serum,the specificity and titer of the antiserum were evaluated by ELISA.High titer of specific antibodies was obtained from the vaccinated mice.The results showed that the GP3 expressed by recombinant baculovirus had strong immunogenicity and could induce high titer antibody against GP3." @default.
- W2362340358 created "2016-06-24" @default.
- W2362340358 creator A5001426883 @default.
- W2362340358 creator A5006746441 @default.
- W2362340358 creator A5026937923 @default.
- W2362340358 creator A5036712663 @default.
- W2362340358 creator A5049009929 @default.
- W2362340358 creator A5077069068 @default.
- W2362340358 creator A5080367913 @default.
- W2362340358 creator A5088171227 @default.
- W2362340358 date "2010-01-01" @default.
- W2362340358 modified "2023-09-26" @default.
- W2362340358 title "Expression of highly pathogenic PRRSV ORF3 gene in insect cells." @default.
- W2362340358 hasPublicationYear "2010" @default.
- W2362340358 type Work @default.
- W2362340358 sameAs 2362340358 @default.
- W2362340358 citedByCount "0" @default.
- W2362340358 crossrefType "journal-article" @default.
- W2362340358 hasAuthorship W2362340358A5001426883 @default.
- W2362340358 hasAuthorship W2362340358A5006746441 @default.
- W2362340358 hasAuthorship W2362340358A5026937923 @default.
- W2362340358 hasAuthorship W2362340358A5036712663 @default.
- W2362340358 hasAuthorship W2362340358A5049009929 @default.
- W2362340358 hasAuthorship W2362340358A5077069068 @default.
- W2362340358 hasAuthorship W2362340358A5080367913 @default.
- W2362340358 hasAuthorship W2362340358A5088171227 @default.
- W2362340358 hasConcept C103878061 @default.
- W2362340358 hasConcept C104317684 @default.
- W2362340358 hasConcept C153911025 @default.
- W2362340358 hasConcept C159047783 @default.
- W2362340358 hasConcept C159654299 @default.
- W2362340358 hasConcept C203014093 @default.
- W2362340358 hasConcept C22744801 @default.
- W2362340358 hasConcept C2522874641 @default.
- W2362340358 hasConcept C2776415932 @default.
- W2362340358 hasConcept C2777773057 @default.
- W2362340358 hasConcept C2779819720 @default.
- W2362340358 hasConcept C2780868878 @default.
- W2362340358 hasConcept C32611913 @default.
- W2362340358 hasConcept C40767141 @default.
- W2362340358 hasConcept C54009773 @default.
- W2362340358 hasConcept C54355233 @default.
- W2362340358 hasConcept C86803240 @default.
- W2362340358 hasConceptScore W2362340358C103878061 @default.
- W2362340358 hasConceptScore W2362340358C104317684 @default.
- W2362340358 hasConceptScore W2362340358C153911025 @default.
- W2362340358 hasConceptScore W2362340358C159047783 @default.
- W2362340358 hasConceptScore W2362340358C159654299 @default.
- W2362340358 hasConceptScore W2362340358C203014093 @default.
- W2362340358 hasConceptScore W2362340358C22744801 @default.
- W2362340358 hasConceptScore W2362340358C2522874641 @default.
- W2362340358 hasConceptScore W2362340358C2776415932 @default.
- W2362340358 hasConceptScore W2362340358C2777773057 @default.
- W2362340358 hasConceptScore W2362340358C2779819720 @default.
- W2362340358 hasConceptScore W2362340358C2780868878 @default.
- W2362340358 hasConceptScore W2362340358C32611913 @default.
- W2362340358 hasConceptScore W2362340358C40767141 @default.
- W2362340358 hasConceptScore W2362340358C54009773 @default.
- W2362340358 hasConceptScore W2362340358C54355233 @default.
- W2362340358 hasConceptScore W2362340358C86803240 @default.
- W2362340358 hasIssue "6" @default.
- W2362340358 hasLocation W23623403581 @default.
- W2362340358 hasOpenAccess W2362340358 @default.
- W2362340358 hasPrimaryLocation W23623403581 @default.
- W2362340358 hasRelatedWork W1966764774 @default.
- W2362340358 hasRelatedWork W1988834150 @default.
- W2362340358 hasRelatedWork W2170126950 @default.
- W2362340358 hasRelatedWork W2347531583 @default.
- W2362340358 hasRelatedWork W2349406542 @default.
- W2362340358 hasRelatedWork W2354951155 @default.
- W2362340358 hasRelatedWork W2355929644 @default.
- W2362340358 hasRelatedWork W2371079755 @default.
- W2362340358 hasRelatedWork W2371339569 @default.
- W2362340358 hasRelatedWork W2371987966 @default.
- W2362340358 hasRelatedWork W2372598617 @default.
- W2362340358 hasRelatedWork W2373432248 @default.
- W2362340358 hasRelatedWork W2376522639 @default.
- W2362340358 hasRelatedWork W2382611472 @default.
- W2362340358 hasRelatedWork W2382830635 @default.
- W2362340358 hasRelatedWork W2385069102 @default.
- W2362340358 hasRelatedWork W2385942962 @default.
- W2362340358 hasRelatedWork W2390720789 @default.
- W2362340358 hasRelatedWork W2887307074 @default.
- W2362340358 hasRelatedWork W3978752 @default.
- W2362340358 hasVolume "32" @default.
- W2362340358 isParatext "false" @default.
- W2362340358 isRetracted "false" @default.
- W2362340358 magId "2362340358" @default.
- W2362340358 workType "article" @default.