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- W2362347029 abstract "Background and purpose:To identify the possibility of Generation 5 Polyamidoamine dendrimers (G5-PAMAM-D) as gene vector for HSV-tk suicide gene therapy in prostate cancer. Methods:Prostrate cancer cells PC-3、22Rv1 were transfected with plasmid pEGFP-C1 coated with G5-PAMAM-D, Furthermore, G5-PAMAM-D were used as gene vectors to deliver plasmid pcDNA3-tk that contain HSV-tk suicide gene into PC-3 and 22Rv1. 48 h after transfection, the precursor ganciclovir(GCV) was added to the transfected PC-3、22Rv1 cells at final concerntration 0, 10,100,1 000, 10 000 μmol/L. MTT was used to evaluate the effect of GCV on the proliferation of two prostate cancer cells after 24 hour incubation. BALB/c mice were subcutaneously injected with PC-3 that was used as tumor model, Polyplex of G5-PAMAM-D and pcDNA3-tk was injected intratumorally followed by GCV injected intraperitoneally 24 hours later. The tumor volume and mice survival time were observed.Results:G5-PAMAM-D could be used as vector to transfer plasmid pEGFP-C1 into two prostate cancer cells, and EGFP was successfully expressed. The number of live cells in G5-PAMAM-D nanoparticles group with HSV-tk is much less than those of the control group, significant difference was found between those two groups. The tumor volume of BALB/c mice was significantly smaller in PAMAM-D/pcDNA3-tk group(1 135±245mm3) than that both in Naked plasmid group(9 965±2 109mm3) and PAMAM-D group(8 357±1 956mm3)(P0.01) 40 days after treatment. Survival time of PAMAM-D/ pcDNA3-tk group was longer than the two control groups.Conclusions:G5-PAMAM-D nanoparticles can deliver HSV-tk gene to prostate cancer cells effectively; it has possibilities of being used as gene vector for gene therapy in prostate cancer." @default.
- W2362347029 created "2016-06-24" @default.
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- W2362347029 date "2007-01-01" @default.
- W2362347029 modified "2023-09-22" @default.
- W2362347029 title "Generation 5 polyamidoamine dendrimers-mediated experimental HSV-tk gene therapy in murine prostate cancer models" @default.
- W2362347029 hasPublicationYear "2007" @default.
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