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- W2362359281 abstract "A peroxidase gene was cloned from alfalfa,which was named as MsPOD.By DNA recombination technology,MsPOD gene was inserted into plasmid pBI121,and a plant expressing vector pBI-POD was constructed.Transgenic Arabidopsis thaliana plants were obtained by floral dip method with Agrobacterium tumefaciens GV3101 harboring pBI-POD plasmid and seeds of transgeinc Arabidopsis thaliana were selected on MS plates containing 50mg/L Kanamycin.PCR analysis of 3 kanamycin resistant plants showed that the target gene was integrated into the genomes of the Arabidopsis thaliana.RT-PCR and GUS staining analysis showed that MsPOD gene expressed in Arabidopsis thaliana." @default.
- W2362359281 created "2016-06-24" @default.
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- W2362359281 date "2013-01-01" @default.
- W2362359281 modified "2023-09-28" @default.
- W2362359281 title "Cloning of MsPODGene from Alfalfa(Medicago sativaL.) and the Transformation of Arabidopsis thaliana" @default.
- W2362359281 hasPublicationYear "2013" @default.
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