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- W2362364098 abstract "Objective To screen appropriate human interleukin-18(hIL-18) mutant and determine its activity. Methods Model the molecular structures of human IL-18 receptor(IL-18R) and IL-18 / IL-18R complex by using protein homology modeling software. Mock construct the three-dimensional structure of IL-18 mutant in which the cysteine at 4 sites were substituted with the other 19 kinds of amino acids by modeler 8.2 software using wild IL-18 as template. Calculate the intermolecular energy of the complex to screen the program for mutation. The mock screened mutants were constructed by molecular biological technique, then expressed, purified and determined for activity. Results A total of 12 mutants were screened according to the theoretical prediction. Both restriction analysis and sequencing proved that the recombinant plasmids containing the mutant genes were constructed correctly. The mutant proteins in forms of inclusion bodies contained about 30% of total somatic protein and reached a purity of more than 90% after purification. The activities of several purified mutants such as C38Glu and C127Ser, at low concentrations, increased slightly, which were basically consistent with those predicted theoretically. Conclusion Several human IL-18 mutants were successfully constructed, which proved that pre-screening by bioinformatic method was helpful to promoting the study on IL-18 mutation." @default.
- W2362364098 created "2016-06-24" @default.
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- W2362364098 date "2008-01-01" @default.
- W2362364098 modified "2023-09-28" @default.
- W2362364098 title "Mock Screening and Activity of Human Interleukin-18 Mutant" @default.
- W2362364098 hasPublicationYear "2008" @default.
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