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- W2362367766 abstract "Aim To study the effect of ATP sensitive potassium channel opener-pinacidil on the apoptosis of PC12 cells induced by ischemia and hypoxia and expression of Bcl-2 mRNA and protein.Methods Cultured PC12 cells were divided into control group,ischemia and hypoxia group,pinacidil and pinacidil+glipizide(ATP sensitive potassium channel blocker) treatment groups. In pinacidil treatment group,pinacidil (100 μmol·L-1) was added 20 min before PC12 cells induced by ischemia and hypoxia;in pinacidil+glipizide treatment group pinacidil (100 μmol·L-1) and glipizide (500 μmol·L-1) were added.Neuronal apoptosis was detected by Annexin-V FITC/PI double-dyed flow cytometry,the expression of Bcl-2 protein was detected by immunofluorescent staining and Western blot.The expression of Bcl-2 mRNA was detected by RT-PCR. Results The apoptotic ratio in ischemia and hypoxia group,pinacidil and pinacidil+glipizide treatment groups was increased with the time passing by.PC12 cells induced by ischemia and hypoxia for 24 hours yielded the highest apoptotic ratio. The apoptotic ratio of pinacidil treatment group is significant less than that in the other groups(P0.01). The expression of Bcl-2 mRNA and protein in ischemia and hypoxia group,pinacidil and pinacidil+glipizide treatment groups were higher at all time points. PC12 cells induced by ischemia and hypoxia for 12 hours yielded the highest. The expression of Bcl-2 mRNA and protein in pinacidil treatment group was significant higher than that of the other groups(P0.05,or P0.01). There were no differences between ischemia and hypoxia group and pinacidil+glipizide treatment group at all time points(P0.05).Conclusions KATP opener can increase the expression of Bcl-2 mRNA and protein,decrease neuronal apoptosis and attenuate the PC12 cell injury caused by ischemia and hypoxia. KATP opener has a protective effect on the apoptosis of PC12 cells induced by ischemia and hypoxia and it is correlated with the up-regulation of the expression of Bcl-2 mRNA and protein." @default.
- W2362367766 created "2016-06-24" @default.
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- W2362367766 date "2009-01-01" @default.
- W2362367766 modified "2023-09-24" @default.
- W2362367766 title "Pinacidil,an ATP-sensitive potassium channel opener,inhibits ischemic apoptosis via up-regulating Bcl-2 expression in PC12 cells" @default.
- W2362367766 hasPublicationYear "2009" @default.
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