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- W2362378107 abstract "To study the phosvitin phosphopeptides(PPP) binding calcium notablely, the raw protein of yolk was used as the material. The optimized technological parameters of double-enzyme fractional hydrolysis with three kinds of enzyme system to prepare PPP were investigated. The optimized conditons using trypsase with one step enzymolysis were that: concentration of substrate 1%, enzyme dosage 5%, pH value 8.0, temperature of hydrolysis 50 ℃ , time of hydrolysis 4 h. The optimized conditions using trypsase and dispase by double-enzyme fractional hydrolysis were that: concentration of substrate 1.4%, pH value 8.0, temperature of hydrolysis 40 ℃, time of hydrolysis 120 min. The optimized conditions using trypsase and Alcalase by double-enzyme fractional hydrolysis were that concentration of substrate 5%, pH value 9.0, temperature of hydrolysis 40 ℃, time of hydrolysis 90 min. It was found that the optimzed enzyme system to prepare PPP was double-enzyme fractional hydrolysis using trypsase and Alcalase, of which the degree of hydrolysis reached 18.12%, the yield 11.49% and the N/P molar ratio 13.91." @default.
- W2362378107 created "2016-06-24" @default.
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- W2362378107 date "2008-01-01" @default.
- W2362378107 modified "2023-09-23" @default.
- W2362378107 title "Experimental research on preparation of phosvitin phosphopeptide by double-enzyme fractional hydrolysis" @default.
- W2362378107 hasPublicationYear "2008" @default.
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