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- W2362412614 abstract "Purpose To establish an enzyme-linked immunosorbent assay for the antisperm antibody detection with combined sperm antigen peptides.Methods Four specific sperm peptides were synthesized by the PS3 automated solid phase peptide synthesizer,purified by RP-HPLC and identified by MS.Based on their antigenicity evaluated by ELISA,the specific sperm peptides were used as coating antigens in the form of combination to detect antisperm antibodies.Results The desired peptides were synthesized,and the purity of these peptides purified by HPLC was over 95%.By comparing the responsibility of the ELISA using combined peptides as coated antigens with commercial kit,a 95.6% coincidence was obtained.Conclusion Solid-phase peptide synthesis could be used to synthesize high purity sperm antigen peptides.The ELISA for the detection of antisperm antibodies using combined sperm antigen peptides as coating antigens can improve the sensitivity." @default.
- W2362412614 created "2016-06-24" @default.
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- W2362412614 date "2010-01-01" @default.
- W2362412614 modified "2023-09-25" @default.
- W2362412614 title "Establishment of an enzyme-linked immunosorbent assay for detecting antisperm antibodies based on sperm antigen peptides" @default.
- W2362412614 hasPublicationYear "2010" @default.
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