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- W2362488441 abstract "Using GoldMag (Fe3O4/Au composite) nanoparticles as a separating medium,a new method for removing the anti-BSA antibody from anti-clenbuterol polyclonal antibody was investigated. As an im-munogenic complex,CBL was conjugated with BSA via diazotized method first,then the polyclonal antibodies were produced in immunized rabbit with CBL-BSA complex. BSA was successfully immobilized onto the GoldMag particles of 50 nm diameter,then the polyclonal antibodies reacted with the BSA on the surface of GoldMag particles and the anti-BSA antibody could be collected on the particle surface,the purified anti-CBL antibody was left in the liquid phase after magnetic separation,the titer of antibodies against CBL and BSA before and after purification was determined by indirect ELISA. The result showed that the immobilization capacity of BSA reached 236μg on 1 mg GoldMag particles by one-step. When the ratio of BSA-GoldMag complex and the polyclonal antibody was 7 :1 and reacted more than 2 hours at 4 C ,the antibodies against BSA can be removed effectively while the titration of antibodies against CBL remained at the same level. This method may become a simple,rapid and effective method for removing the unrelated antibodies in polycolnal antibodies against small molecules." @default.
- W2362488441 created "2016-06-24" @default.
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- W2362488441 date "2006-01-01" @default.
- W2362488441 modified "2023-09-25" @default.
- W2362488441 title "Purification of anti-clenbuterol hydrochloride polyclonal antibody by GoldMag nanoparticles" @default.
- W2362488441 hasPublicationYear "2006" @default.
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