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- W2362508740 abstract "Objective Alterations of protein expression in cytosol and extracellular fluid and exchanges between intracellular and extracellular proteins of thymocytes in mice 4 hours after whole body irradiation with 75 mGy X rays were investigated.Methods Two dimensional electrophoresis was used.Results It was found that Alterations of 12 proteins ranging in size from 10 to 53 kD with their pI distribution from 5.0 to 8.2 were displayed in the cytoplasmic fraction of thymocytes in irradiated mice,of which there were 5 novel proteins,4 upregulated proteins and 3 down regulated proteins,and 5 proteins ranging in size from 32 to 67 kD with their pI distribution from 5.8 to 7.7 disappeared after irradiation.Alterations of 3 proteins ranging in size from 15 to 67 kD with their pI distribution from 5.1 to 8.2 were displayed in the extracellular fluid of thymocytes in irradiated mice,of which there were one novel protein,one up regulated protein and one down regulated protein and 3 proteins ranging in size from 13 to 69 kD with their pI distribution from 5.0 to 8.0 disappeared after irradiation.It was shown that a protein molecule in the sham irradiated cytosol of thymocytes appeared in the extracellular fluid after irradiation and a protein molecule in the sham irradiated cytosol was decreased after irradiation and increased in the extracellular fluid.Two proteins in sham irradiated extracellular fluid appeared in the thymocyte cytosol after irradiation.Conclusion The results suggest that alterations of protein expression and exchanges of protein molecules occurred between intracellular and extracellular compartments after irradiation.These findings may have implications in the mechanism of immunoenhancement and adaptive response induced by low dose radiation.The elucidation of the nature and mode of action of these protein molecules remains to be clarified in future studies." @default.
- W2362508740 created "2016-06-24" @default.
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- W2362508740 date "2000-01-01" @default.
- W2362508740 modified "2023-09-25" @default.
- W2362508740 title "Effect of ionizing radiation on protein expression in murine thymocytes" @default.
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