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- W2362511360 abstract "To construct the eukaryotic vector pcDNA3.1(-)-SQR-Myc and investigate its expression in eukaryocyte,the 5′ primers and 3′ primers were designed and synthesized following the gene sequence of SQR from Rhodobacter capsulatus.The sulfide-quinone reductase(SQR)gene was obtained by PCR and the intermediate vector pMD-18T-SQR-Myc was constructed.The vector was then digested by restriction enzymes XhoⅠand KpnⅠ,and the SQR gene was inserted into the the corresponding restriction enzyme cutting site to construct recombinant plasmid pcDNA3.1(-)-SQR-Myc.The plasmid pcDNA3.1(-)-SQR-Myc was transiently transfected into the HEK293 cells with lipidosome and identified by Western blotting.The results showed that the eukaryotic vector pcDNA3.1(-)-SQR-Myc was constructed successfully,which was confirmed by restriction enzyme digestion and sequencing.Western blotting revealed that SQR could be expressed in HEK293 cells." @default.
- W2362511360 created "2016-06-24" @default.
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- W2362511360 date "2011-01-01" @default.
- W2362511360 modified "2023-09-23" @default.
- W2362511360 title "Construction and expression of eukaryotic vector of sulfide-quinone reductase(SQR) gene" @default.
- W2362511360 hasPublicationYear "2011" @default.
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