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• W2362532912 abstract "OBJECTIVE To identify the type of the intermediate filament (IF) protein of Angiostrongylus cantonensis and analyze its tissue localization. METHODS Recombinant pET-IF of antigen IF was expressed in E.coli with IPTG induction, and the expression products were purified by His.Bind column and identified for determining the type of the IF protein by Western blotting. Anti-IF antibody was prepared by multi-spot subcutaneous injection into mouse and used to detect the tissue slices of A. cantonensis by immunohistochemical analysis. RESULTS The antigen IF were correctly expressed and purified, and identified as a keratin located in the intestine wall and cytoplusma. CONCLUSION The antigen IF is distributed in the intestine wall of A. cantonensis." @default.
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• W2362532912 date "2007-08-01" @default.
• W2362532912 modified "2023-09-24" @default.
• W2362532912 title "Identification and tissue localization of intermediate filament protein in Angiostrongylus Cantonensis" @default.
• W2362532912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17715010" @default.
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