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- W2362562014 abstract "The increasing availability of expressed sequence tags(ESTs) sequences provides an invaluable resource of EST-SSR markers.The EST sequences encoding transcription used in this study included 6951 ESTs with SSR repeats downloaded from NCBI databases.A total of 4338 microsatellites(SSRs) were isolated from the 6951 sugarbeet expressed sequence tags(ESTs).According to primer design criteria,2845 primer pairs for EST-SSR were designed.Under an appropriate polymerase chain reaction(PCR) system,randomly synthesized 100 EST-SSR primer pairs were screened against genomic DNA of 6 sugarbeet varieties,and 47 EST-SSR primer pairs out of the above 100 resulted in PCR products.Then,all 47 pairs were detected in six sugarbeet varieties for polymorphisms,three native and three foreign cultivars were clustered two groups,and 25 pairs(53.2%) were found to be polymorphic,accounting for 0.97% of the total number of designed primer pairs.Those results demonstrate the possibility of developing EST-SSR markers using sugarbeet EST-SSR markers." @default.
- W2362562014 created "2016-06-24" @default.
- W2362562014 creator A5083130438 @default.
- W2362562014 date "2011-01-01" @default.
- W2362562014 modified "2023-09-26" @default.
- W2362562014 title "Development and Utilization of EST-SSR Marker in Sugarbeet(Beta vulgaris L.)" @default.
- W2362562014 hasPublicationYear "2011" @default.
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