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- W2362580393 abstract "Objective To study the function of HCV-specific cytotoxic T lymphocytes (HCV-CTL) and its influencing factors in patients with chronic hepatitis C.Methods A cell line expressing stably HCV core protein-HepG2-core was constructed by gene transfecting the plasmid pcDNA3.1-core (expressing HCV core protein) into HepG2 by Lipofecta- mineTM. HepG2 cells transfected with recombinant eukaryotic expression plasmid were selected by G418. The HCV-C protein in HepG2 cells transfected with recombinant plasmid was verified by Western blot assay. HCV-CTL got from PBMC and stimulated by HCV antigen peptide, activity of HCV-CTL were tested by LDH releasing assay, the levels of IFN-γ of cells culture were detected by ELISA assay. 22 HLA-A2 positive patients with chronic hepatitis C were tested and 8 healthy subjects as controls.Results Activity (23.9±4.8)% of HCV-specific CTL of patients with chronic hepatitis C was significant lower than that (42.6±6.5)% of the normal subjects (t=7.22, P=0.011). Activity (18.9±4.8)% of HCV-specific CTL of patients with high viral load was significant lower than that (33.7±3.2)% of the patients with low viral load (t=8.22, P=0.003). Activity (20.8±2.1)% of HCV-specific CTL of patients with genotype 1 was significant lower than that (32.4±2.5)% of the patients with genotype 2 or 3(t=11.7,P=0.001). Activity (29.3±3.1)% of HCV-specific CTL of patients with high levels of serum ALT was no significant difference with that (25.7±3.4)% of the patients with normal levels of ALT(t=0.93, P0.05). The concentration (957±241 )pg/ml of IFN-γ of cells culture of patients with chronic hepatitis C was significant lower than that (3 117±673)pg/ml of controls (t=8.87, P=0.001). Conclusion The activity of HCV-CTL of patients with chronic hepatitis C was low, which was related with concentration of HCV-RNA and genotype, but not the levels of ALT." @default.
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- W2362580393 title "Study of the function of HCV-specific cytotoxic T lymphocytes in chronic hepatitis C" @default.
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