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- W2362582444 abstract "Objective To study the effect of riboflavin(RF) photochemical method on E.coli in plasma using 313nm wavelength combined with vitamin C(Vc) to excite light in different oxygenic environments.Methods 50μmol/L RF and(0,12.5,25 or 37.5mmol/L) of Vc in pooled plasma were mixed with intesfinel E.coli.The mixture was irradiated by 313nm wavelength light in 12 well cultured plates or PVC blood storage bags(oxygen environment or hypoxic environment).The dose of irradiation was 5J/mL.The inactivation effects on E.coli were observed in different oxygen environments.Results Vc promoted photochemical inactivation of E.coli in 12 well cultured plates.However,the promotion effect of Vc was not obvious in PVC storage bag(due to lack of oxygen).Compared with the hypoxic environment,Vc was more likely to promote the RF photochemical inactivation of E.coli in the blood storage bag filled with pure oxygen.RF was then irradiated with 313nm wavelength,combined with 10mmol/L Vc,which led to a decrease in titer of E.coli by 4.3log/mL.Conclusion In an environment with high oxygen levels,the combination of RF and Vc irradiated by 313nm wavelength light source can effectively inactivate E.coli in plasma.Vc has the possibility to be one of the photochemical substrates used for pathogen inactivation." @default.
- W2362582444 created "2016-06-24" @default.
- W2362582444 creator A5085639385 @default.
- W2362582444 date "2015-01-01" @default.
- W2362582444 modified "2023-09-26" @default.
- W2362582444 title "The inactivation of Escherichia coli using riboflavin photochemistry combined with vitamin C in different oxygenic atmosphere" @default.
- W2362582444 hasPublicationYear "2015" @default.
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