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- W2362583145 abstract "A high performance liquid chromatographic method is described for the determination of ten biogenic amines found in aquatic products: tryptamine,octopamine,2-phenylethylamine,putrescine,cadaverine,histamine,5-hydroxytryptamine,tyramine,spermidine,and spermine.Amines were extracted with perchloric acid solution and derived with dansyl chloride.After derivation, ammonia was added to remove an interfering peak near cadaverine.Liquid chromatographic separations were performed by using a Spherisorb ODS2 column and an ammonium acetate-acetonitrile gradient elution program with mixture of0.1 mol/L ammonium acetate as solvent A and acetonitrile as solvent B.Flow rate is 1.0 mL/min and column temperature is 40 ℃.UV detector is used to determine the biogenic amines at 254 nm wavelength.Average recovery of biogenic amines ranges from 79.59% to 111.10%.Relative standard deviations range from 2.6% to 15.8%. The limits of detection(S/N=3) were: 0.8 μg/g for putrescine and spermidine;1 μg/g for cadaverine,histamine,tyramine and spermine;2 μg/g for octopamine and serotonin;3 μg/g for 2-phenylethylamine;5 μg/g for tryptamine." @default.
- W2362583145 created "2016-06-24" @default.
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- W2362583145 date "2007-01-01" @default.
- W2362583145 modified "2023-09-23" @default.
- W2362583145 title "Determination of biogenic amines in aquatic products" @default.
- W2362583145 hasPublicationYear "2007" @default.
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