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- W2362600963 abstract "Objective Expression of human single chain Fv antibody to hepatitis B core protein in E. coli. Methods The phage antibody library was panned by HBc antigen which was coated in a micro-titer plate, after five rounds of bio-panning, 60 clones were identified specific to HBc antigen. The specificity of ScFv was determined by ELISA. E.coli HB2151 were transformed and induced by IPTG. The specificity of ScFv was evaluated by ELISA and western blot hybridization. ResultsHBc-ScFv DNA digestion and sequence data showed that the ScFv gene is composed of 771 bp. ELISA and western blot demonstrated that the soluble human single chain Fv antibody to hepatitis B virus core antigen has a specific combination character with hepatitis B virus core ant igen. Conclusion Identification and expression of HBc antigen specific ScFv from E.coli were successfully achieved." @default.
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- W2362600963 date "2002-01-01" @default.
- W2362600963 modified "2023-09-23" @default.
- W2362600963 title "Expression of soluble humansingle chain Fv antibody to hepatitis B core antigen in E. coli" @default.
- W2362600963 hasPublicationYear "2002" @default.
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