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- W2362656049 abstract "Objective To construct the yeast expression vector of HBV preS1 gene for investigating the potential role of preS1 protein in mediating the attachment of HBV particles to human hepatocytes.Methods PCR was performed to amplify HBV preS1 gene from the plasmid PCP10/HBV ayw subtype containing the whole fragment of HBV,and the PCR product was cloned into pGEM-T vector and then named T-preS1.HBV preS1 gene was cut from T-preS1 by NcoⅠ and MluⅠ,and cloned into yeast expression plasmid pSos;the reconstructed plasmid was tested by auto-sequencing assay and named pSos-preS1.pSos-preS1 was transformed into yeast cell cdc25(a) by LiAc mediated transformation,and the yeast protein was isolated and analyzed by Western blot.Results The yeast expression vector of HBV preS1 gene was constructed successfully,and the presence of HBV preSl protein in yeast cells was confirmed by Western blot analysis.Conclusion Reconstruction of pSos-preS1 has laid a foundation for better understanding of the mechanism of HBV preS1 protein in viral endocytosis and is helpful in seeking the preS1 related protein that is supposed to interact with preS1 protein in vitro by sos-recruitment system." @default.
- W2362656049 created "2016-06-24" @default.
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- W2362656049 date "2009-01-01" @default.
- W2362656049 modified "2023-09-23" @default.
- W2362656049 title "Construction and expression of HBV preS1 gene in yeast" @default.
- W2362656049 hasPublicationYear "2009" @default.
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