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- W2362657703 abstract "Objective:To establish a method for the chiral separations of glutamic acid(Glu)and aspartic acid (Asp)by high performance liquid chromatography(HPLC).Method:The amino acids were derivatized with o- phthaladehyde(OPA)before separations.β-CD was used as a chiral mobile phase additive.The mobile phase was acetonitrile-buffer solution [0.02 mol·L~(-1)NaAc-(CH_3CH_2)_3 N=100:0.018].The detective wavelength was 330 nm.The influences of colums,the composition of mobile phase,pH,the concentration of β-CD and colum temperature on the enantiomerie separations of Glu and Asp were investigated.The optimized chromatographic con- ditions for the separations were at 40℃ on a Hypersil AA-ODS colum and the mobile phase was acetonitrile- buffer solution [0.02 mol·L~(-1)NaAc-(CH_3 CH_2)_3 N=100:0.018,adjusting to pH=7.2 with 2% HAc and con- taining 6 mmol·L~(-1)β-CD](1:99)at a flow rate of 0.45 mL·min~(-1).Results:Under the chromatographic con- ditions,baseline separations of these enantiomers could be obtained.Conclusion:The method was simple,rapid with high resolutions." @default.
- W2362657703 created "2016-06-24" @default.
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- W2362657703 date "2006-01-01" @default.
- W2362657703 modified "2023-09-25" @default.
- W2362657703 title "RP-HPLC chiral separations of glutamic acid and aspartic acid" @default.
- W2362657703 hasPublicationYear "2006" @default.
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