Matches in SemOpenAlex for { <https://semopenalex.org/work/W2362787777> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2362787777 abstract "To get high concentrative antibody,specifically for Western blot of VMP1,VMP1 gene was cloned from the L929 cell line by RT-PCR and recombined into the vector of pCMV5-myc.PCR was used to amplify the DNA fragment corresponding to the amino acid 132-239 sequence of VMP1 from pCMV5-myc-VMP1.Then the fragment was ligased to pGST-parallel vector,overexpressed in The E coli.strain BL21.The protein was purified by GST beads,then immuned rabbits.9 weeks later,the serum of rabbits was harvested and anti-VMP1 polyclonal antibodies was purified.VMP1 was successfully detected by western blot with anti-VMP1 antibody in 293T cells transfected with pCMV5-myc-VMP1 and endogenously in mouse L929 cell line.The results show that the antibody is high concentrative with good immunological characteristics.Antibody with high concentration and specificity can be well obtained by this method,which most lAbs can afford.Furthermore,the antibody lays a good foundation for further research on the function of VMP1 in cell,especially on its role in signal transduction pathway." @default.
- W2362787777 created "2016-06-24" @default.
- W2362787777 creator A5057644492 @default.
- W2362787777 date "2006-01-01" @default.
- W2362787777 modified "2023-09-27" @default.
- W2362787777 title "Preparation and Identification of the Rabbit Polyclonal Antibodies from VMP1" @default.
- W2362787777 hasPublicationYear "2006" @default.
- W2362787777 type Work @default.
- W2362787777 sameAs 2362787777 @default.
- W2362787777 citedByCount "0" @default.
- W2362787777 crossrefType "journal-article" @default.
- W2362787777 hasAuthorship W2362787777A5057644492 @default.
- W2362787777 hasConcept C104317684 @default.
- W2362787777 hasConcept C153911025 @default.
- W2362787777 hasConcept C159047783 @default.
- W2362787777 hasConcept C159654299 @default.
- W2362787777 hasConcept C185592680 @default.
- W2362787777 hasConcept C2776415932 @default.
- W2362787777 hasConcept C54009773 @default.
- W2362787777 hasConcept C54355233 @default.
- W2362787777 hasConcept C55493867 @default.
- W2362787777 hasConcept C86803240 @default.
- W2362787777 hasConcept C956191 @default.
- W2362787777 hasConceptScore W2362787777C104317684 @default.
- W2362787777 hasConceptScore W2362787777C153911025 @default.
- W2362787777 hasConceptScore W2362787777C159047783 @default.
- W2362787777 hasConceptScore W2362787777C159654299 @default.
- W2362787777 hasConceptScore W2362787777C185592680 @default.
- W2362787777 hasConceptScore W2362787777C2776415932 @default.
- W2362787777 hasConceptScore W2362787777C54009773 @default.
- W2362787777 hasConceptScore W2362787777C54355233 @default.
- W2362787777 hasConceptScore W2362787777C55493867 @default.
- W2362787777 hasConceptScore W2362787777C86803240 @default.
- W2362787777 hasConceptScore W2362787777C956191 @default.
- W2362787777 hasLocation W23627877771 @default.
- W2362787777 hasOpenAccess W2362787777 @default.
- W2362787777 hasPrimaryLocation W23627877771 @default.
- W2362787777 hasRelatedWork W2348991363 @default.
- W2362787777 hasRelatedWork W2354466098 @default.
- W2362787777 hasRelatedWork W2371728522 @default.
- W2362787777 hasRelatedWork W2377741989 @default.
- W2362787777 hasRelatedWork W2399188697 @default.
- W2362787777 hasRelatedWork W2400127642 @default.
- W2362787777 hasRelatedWork W2404970265 @default.
- W2362787777 hasRelatedWork W2408761790 @default.
- W2362787777 hasRelatedWork W2414000778 @default.
- W2362787777 hasRelatedWork W2414179442 @default.
- W2362787777 hasRelatedWork W2415911233 @default.
- W2362787777 hasRelatedWork W2946974237 @default.
- W2362787777 hasRelatedWork W2989981113 @default.
- W2362787777 hasRelatedWork W2995425410 @default.
- W2362787777 hasRelatedWork W3025170506 @default.
- W2362787777 hasRelatedWork W3031072009 @default.
- W2362787777 hasRelatedWork W3044262056 @default.
- W2362787777 hasRelatedWork W3146540107 @default.
- W2362787777 hasRelatedWork W75010430 @default.
- W2362787777 hasRelatedWork W2875310867 @default.
- W2362787777 isParatext "false" @default.
- W2362787777 isRetracted "false" @default.
- W2362787777 magId "2362787777" @default.
- W2362787777 workType "article" @default.