Matches in SemOpenAlex for { <https://semopenalex.org/work/W2362801886> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2362801886 abstract "Objective In order to improving the efficiency in the research of the rice genetics breeding using SSR marker the methods of DNA rapid extraction from single rice seedling and rapid sliver staining after PAGE were developed. Methods 10 mg fresh single rice seedling within a 1.5 mL EP tube was grounded rapidly with 100 μL extraction buffer at room temperature. Immediately after grounding 300 μL extraction buffer was added to the tube. Then the extracts was incubated in a water bath at 65℃ for 10 min. After centrifugation precipitation with pure ethanol dry under a weak vacuum dissolved by TE it could be applied to PCR. The improved method of PAGE sliver staining needed three steps which meant staining rinsing and developing. Only ethanol and silver nitrate solution were used in staining. After rinsing gels with deionized water it could transfer into 500 mL developer solution to develop. Results Results revealed that the method need no liquid nitrogen no octanol-chloroform the main band of DNA extracted by the rapid method was clear and slightly degraded indicating that it could be used to SSR-PCR analysis. The simple and rapid method of PAGE sliver staining was taken only about 10 min to staint the gels and the background of gels was bright high sensibility. Conclusion With adoption of SSR marker techniques the rapid method of DNA extraction and PAGE sliver staining could be effectively applied to molecular marker detection of F2 segregant population and supplementary screen of breed materials." @default.
- W2362801886 created "2016-06-24" @default.
- W2362801886 creator A5005090069 @default.
- W2362801886 date "2006-01-01" @default.
- W2362801886 modified "2023-09-25" @default.
- W2362801886 title "An Improved Rapid DNA Extraction from Single Rice Seedling and PAGE Sliver Staining Method" @default.
- W2362801886 hasPublicationYear "2006" @default.
- W2362801886 type Work @default.
- W2362801886 sameAs 2362801886 @default.
- W2362801886 citedByCount "0" @default.
- W2362801886 crossrefType "journal-article" @default.
- W2362801886 hasAuthorship W2362801886A5005090069 @default.
- W2362801886 hasConcept C104317684 @default.
- W2362801886 hasConcept C13965031 @default.
- W2362801886 hasConcept C144024400 @default.
- W2362801886 hasConcept C149923435 @default.
- W2362801886 hasConcept C185592680 @default.
- W2362801886 hasConcept C2776096895 @default.
- W2362801886 hasConcept C2778476535 @default.
- W2362801886 hasConcept C2780472540 @default.
- W2362801886 hasConcept C2908647359 @default.
- W2362801886 hasConcept C43617362 @default.
- W2362801886 hasConcept C4725764 @default.
- W2362801886 hasConcept C49105822 @default.
- W2362801886 hasConcept C54355233 @default.
- W2362801886 hasConcept C55493867 @default.
- W2362801886 hasConcept C59822182 @default.
- W2362801886 hasConcept C74864618 @default.
- W2362801886 hasConcept C7623868 @default.
- W2362801886 hasConcept C78063203 @default.
- W2362801886 hasConcept C86803240 @default.
- W2362801886 hasConceptScore W2362801886C104317684 @default.
- W2362801886 hasConceptScore W2362801886C13965031 @default.
- W2362801886 hasConceptScore W2362801886C144024400 @default.
- W2362801886 hasConceptScore W2362801886C149923435 @default.
- W2362801886 hasConceptScore W2362801886C185592680 @default.
- W2362801886 hasConceptScore W2362801886C2776096895 @default.
- W2362801886 hasConceptScore W2362801886C2778476535 @default.
- W2362801886 hasConceptScore W2362801886C2780472540 @default.
- W2362801886 hasConceptScore W2362801886C2908647359 @default.
- W2362801886 hasConceptScore W2362801886C43617362 @default.
- W2362801886 hasConceptScore W2362801886C4725764 @default.
- W2362801886 hasConceptScore W2362801886C49105822 @default.
- W2362801886 hasConceptScore W2362801886C54355233 @default.
- W2362801886 hasConceptScore W2362801886C55493867 @default.
- W2362801886 hasConceptScore W2362801886C59822182 @default.
- W2362801886 hasConceptScore W2362801886C74864618 @default.
- W2362801886 hasConceptScore W2362801886C7623868 @default.
- W2362801886 hasConceptScore W2362801886C78063203 @default.
- W2362801886 hasConceptScore W2362801886C86803240 @default.
- W2362801886 hasLocation W23628018861 @default.
- W2362801886 hasOpenAccess W2362801886 @default.
- W2362801886 hasPrimaryLocation W23628018861 @default.
- W2362801886 hasRelatedWork W1984303133 @default.
- W2362801886 hasRelatedWork W2349656013 @default.
- W2362801886 hasRelatedWork W2356458831 @default.
- W2362801886 hasRelatedWork W2358493451 @default.
- W2362801886 hasRelatedWork W2360506994 @default.
- W2362801886 hasRelatedWork W2366137337 @default.
- W2362801886 hasRelatedWork W2366173831 @default.
- W2362801886 hasRelatedWork W2380832720 @default.
- W2362801886 hasRelatedWork W2383073227 @default.
- W2362801886 hasRelatedWork W2384689745 @default.
- W2362801886 hasRelatedWork W2385973432 @default.
- W2362801886 hasRelatedWork W2389473730 @default.
- W2362801886 hasRelatedWork W2390141694 @default.
- W2362801886 hasRelatedWork W2393703091 @default.
- W2362801886 hasRelatedWork W2512430663 @default.
- W2362801886 hasRelatedWork W2850266580 @default.
- W2362801886 hasRelatedWork W2852756915 @default.
- W2362801886 hasRelatedWork W2870623338 @default.
- W2362801886 hasRelatedWork W2926307451 @default.
- W2362801886 hasRelatedWork W2966456089 @default.
- W2362801886 isParatext "false" @default.
- W2362801886 isRetracted "false" @default.
- W2362801886 magId "2362801886" @default.
- W2362801886 workType "article" @default.