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• W2362829420 abstract "A strain of Lyme disease Borrelia burgdorferi was isolated from field ticks collected in Shangzhi Prefecture of Heilongjiang Province,and its morphology and molecular characteristics were studied.Helical structure of the cultured bacterium in BSK H medium was observed on Giemsa stained slides under microscope with dark field.Genomic DNA of the strain was amplified by 16S rRNA and OspA gene based PCR,and the PCR products were sequenced.The sequences of 16S rRNA and OspA gene were compared to that of other Borrelia burgdorferi strains available in GenBank.Two phylogenetic trees were constructed.The results revealed that the 16S rRNA gene sequence shared 99.5% similarity to that of Borrelia garinii strain DK27(X85193),and the OspA gene sequence shared 93.6% similarity to that of B.garinii strain Khab(AY502600).According to the sequences analysis,the strain could be identified to be B.garinii,and named SZ.The sequences have been submitted to GenBank(Accession No.:HM007279,HM007278)." @default.
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• W2362829420 date "2010-01-01" @default.
• W2362829420 modified "2023-09-25" @default.
• W2362829420 title "Identification and phylogenetic analysis of Lyme disease Borrelia spp. isolated from Shangzhi Prefecture of Heilongjiang Province, China." @default.
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