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- W2362839416 abstract "AIM: To investigate the refolding of the reduced/denatured ribonuclease (RNase) by high performance hydrophobic interaction chromatography (HPHIC) and high performance ion exchange chromatography (HPIEC) and to study the factors influencing its refolding. METHODS: The reduced/denatured RNase by guanidine hydrochloride or urea with high concentration in the presence of dithiotheitol (DTT) was first oxidized by oxidized-gluthatione (GSSG) in solution and then refolded by liquid chromatography. RESULTS: In the presence of low concentration of denaturing agent and a suitable concentration of GSSG in the mobile phrase, the bioactivity and mass recoveries of the refolded RNase increased. CONCLUSION: Compared with other elution methods, both HPHIC and HPIEC, with the advantages of high renaturation efficiency, fast process and simple operation, have good potentials in application." @default.
- W2362839416 created "2016-06-24" @default.
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- W2362839416 date "2005-01-01" @default.
- W2362839416 modified "2023-09-23" @default.
- W2362839416 title "Refolding of reduced/denatured ribonuclease by high performance liquid chromatography" @default.
- W2362839416 hasPublicationYear "2005" @default.
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