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- W2362914963 abstract "Objective To establish a GFP labeling human Colon cancer cell line and to generate a noninvasive model for monitoring tumor growth in vivo.Method Human Colon cancer cells were transfected with chicken β-actin-GFP-NEO by lipofectin 2000 reagent and screened with G418 to produce the stable GFP-expressing clone.Then the labeled HCT-8 cells were implanted into the subcutaneous tissue of nude mice and the growth of the implanted tumor was observed and growth curve of the labeled HCT-8 cells was analyzed with the whole-body optical imaging system.Result The stable GFP-expressing HCT-8 cell lines were obtained,the growth of the tumor was observed after subcutaneous inoculation of labeled HCT-8 to BALB/AC-nu.Conclusion A GFP-labeled HCT-8 cell line and a mouse tumor model based on the cell line were established,which is a convenient and useful in vivo model for the research on human colon cancer and drug discovery." @default.
- W2362914963 created "2016-06-24" @default.
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- W2362914963 date "2009-01-01" @default.
- W2362914963 modified "2023-09-26" @default.
- W2362914963 title "The Establishment of Human Colon Cancer Model Labeled by GFP" @default.
- W2362914963 hasPublicationYear "2009" @default.
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