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- W2363017568 abstract "Objective To study the various influ- ent factors on the growth rate of laryngeal carcinoma celi culture in vitro and to provide the experiment base for the establishment of human laryngeal squamous carcinoma celi lines. Methods Applying the technique of primary tissue culture in vitro, twenty-four specimens, which were identified pathologically as laryngeal squa-mous cell carcinoma, were cultured. The relation among the cell growth rate and donor age, the differentiation of the carcinoma cells, cultured methods were observed. The influent factors of primary culture, such as fibrob-last and micro biotic contamination were analyzed. Results Cell growth rate were 31.25% (5/16) inthe group aged under 60, 37.5% in the group above 60, 100% for the well differentiation, 30.8% for the mild differentiation and 25% for the poor differentiation. The cell growth rate were 43.75% in tumor tissue culture and 10% in enzvme digestion culture. The epithelia spread from the tissue in 5(7 days. To all specimens, fibroblast outgrowth and micro biotic contamination were the im-portant factors in obstacle the growth of squamous cell carcinoma. Conclusions The cell growth of laryngeal squamous cell carcinoma in vitro could be correlated with the tumor differentiation and the culture methods; fibroblast outgrowth and micro biotic contamination are important factors to obstacle the establishment of laryn-geal squamous cell carcinoma." @default.
- W2363017568 created "2016-06-24" @default.
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- W2363017568 date "2003-01-01" @default.
- W2363017568 modified "2023-09-24" @default.
- W2363017568 title "A tentative study on primary culture in vitro of twenty-four laryngeal squamous carcinoma tissue" @default.
- W2363017568 hasPublicationYear "2003" @default.
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