Matches in SemOpenAlex for { <https://semopenalex.org/work/W2363060775> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2363060775 abstract "Objective Knockout mice are widely used in the studies of joint diseases. This article investigated the effects of joint processing methods,collagenase types,and collagenase digestion time on the number of primary fibroblast-like synoviocytes( FLSs) obtained from mice. Methods The hind legs of 6 of the 12 male mice were cut open from the hip joints,but not those of the other 6. FLSs were isolated using the type-Ⅳ collagenase digestion method and purified by differential digestion. Cell morphology was observed under the inverted microscope. The type,viability,and purity of the cells were determined by flow cytometry. Results Significantly fewer FLSs were obtained from the mice with the hind legs cut open( 19 133 ± 115) than from those without( 24 933 ±503)( P 0. 05). The numbers of FLSs collected from the cell suspension at 1,2,3,4,5,6,and 7 hours after digestion were700 ± 300,600 ± 100,15 200 ± 900,5100 ± 800,2700 ± 300,900 ± 200,and 300 ± 100,respectively,the highest at 3 hours.There were statistically significant differences in the total number of FLSs obtained by type-Ⅳ and type-Ⅱ collagenase digestions( 24900 ±500 vs 18100 ± 400,P 0. 05). Conclusion For in vitro culture of primary mouse FLSs,it is recommended that the hip joints be not cut open,and type-Ⅳ collagenase be used with cell suspension at 2-6 hours after digestion." @default.
- W2363060775 created "2016-06-24" @default.
- W2363060775 creator A5082647057 @default.
- W2363060775 date "2014-01-01" @default.
- W2363060775 modified "2023-09-24" @default.
- W2363060775 title "Influencing factors on the primary culture of mouse fibroblast-like synoviocytes" @default.
- W2363060775 hasPublicationYear "2014" @default.
- W2363060775 type Work @default.
- W2363060775 sameAs 2363060775 @default.
- W2363060775 citedByCount "0" @default.
- W2363060775 crossrefType "journal-article" @default.
- W2363060775 hasAuthorship W2363060775A5082647057 @default.
- W2363060775 hasConcept C134018914 @default.
- W2363060775 hasConcept C1491633281 @default.
- W2363060775 hasConcept C153911025 @default.
- W2363060775 hasConcept C16685009 @default.
- W2363060775 hasConcept C181199279 @default.
- W2363060775 hasConcept C185592680 @default.
- W2363060775 hasConcept C202751555 @default.
- W2363060775 hasConcept C2778394429 @default.
- W2363060775 hasConcept C2780381497 @default.
- W2363060775 hasConcept C2780644872 @default.
- W2363060775 hasConcept C2781357212 @default.
- W2363060775 hasConcept C43617362 @default.
- W2363060775 hasConcept C53227056 @default.
- W2363060775 hasConcept C54355233 @default.
- W2363060775 hasConcept C553184892 @default.
- W2363060775 hasConcept C55493867 @default.
- W2363060775 hasConcept C71924100 @default.
- W2363060775 hasConcept C81885089 @default.
- W2363060775 hasConcept C86803240 @default.
- W2363060775 hasConceptScore W2363060775C134018914 @default.
- W2363060775 hasConceptScore W2363060775C1491633281 @default.
- W2363060775 hasConceptScore W2363060775C153911025 @default.
- W2363060775 hasConceptScore W2363060775C16685009 @default.
- W2363060775 hasConceptScore W2363060775C181199279 @default.
- W2363060775 hasConceptScore W2363060775C185592680 @default.
- W2363060775 hasConceptScore W2363060775C202751555 @default.
- W2363060775 hasConceptScore W2363060775C2778394429 @default.
- W2363060775 hasConceptScore W2363060775C2780381497 @default.
- W2363060775 hasConceptScore W2363060775C2780644872 @default.
- W2363060775 hasConceptScore W2363060775C2781357212 @default.
- W2363060775 hasConceptScore W2363060775C43617362 @default.
- W2363060775 hasConceptScore W2363060775C53227056 @default.
- W2363060775 hasConceptScore W2363060775C54355233 @default.
- W2363060775 hasConceptScore W2363060775C553184892 @default.
- W2363060775 hasConceptScore W2363060775C55493867 @default.
- W2363060775 hasConceptScore W2363060775C71924100 @default.
- W2363060775 hasConceptScore W2363060775C81885089 @default.
- W2363060775 hasConceptScore W2363060775C86803240 @default.
- W2363060775 hasLocation W23630607751 @default.
- W2363060775 hasOpenAccess W2363060775 @default.
- W2363060775 hasPrimaryLocation W23630607751 @default.
- W2363060775 hasRelatedWork W1985606434 @default.
- W2363060775 hasRelatedWork W1989322592 @default.
- W2363060775 hasRelatedWork W2024667287 @default.
- W2363060775 hasRelatedWork W2033590953 @default.
- W2363060775 hasRelatedWork W2080091182 @default.
- W2363060775 hasRelatedWork W2136947287 @default.
- W2363060775 hasRelatedWork W2347590573 @default.
- W2363060775 hasRelatedWork W2352706065 @default.
- W2363060775 hasRelatedWork W2353225338 @default.
- W2363060775 hasRelatedWork W2354298053 @default.
- W2363060775 hasRelatedWork W2356678622 @default.
- W2363060775 hasRelatedWork W2364772211 @default.
- W2363060775 hasRelatedWork W2365887819 @default.
- W2363060775 hasRelatedWork W2367189688 @default.
- W2363060775 hasRelatedWork W2377997093 @default.
- W2363060775 hasRelatedWork W2380732568 @default.
- W2363060775 hasRelatedWork W2386286251 @default.
- W2363060775 hasRelatedWork W2390626322 @default.
- W2363060775 hasRelatedWork W2560824688 @default.
- W2363060775 hasRelatedWork W757567435 @default.
- W2363060775 isParatext "false" @default.
- W2363060775 isRetracted "false" @default.
- W2363060775 magId "2363060775" @default.
- W2363060775 workType "article" @default.