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- W2363090429 abstract "n amplified fragment of 245 base pairs from IS-986 sequence hybridized specifically with DNA of M·tuberculosis complex strains. The fragment was la-belled with digoxigenin. after amplification by PCRand southern blot hybridization with the dig-labelledprobe,the limit of detection of purified genomic M. tu-berculosis DNA amounted to 1 fg. We detected spu-tum,pleural and synovial fluid with active tuberculosisby PCR and southern blot, and the sensitivities were81.0%, 64.2%and 57.7%,respectively.From theresult of the experiment,it is a very useful method fordiagnosis of tuberculosis by combining PCR withSouthern blot." @default.
- W2363090429 created "2016-06-24" @default.
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- W2363090429 date "1994-01-01" @default.
- W2363090429 modified "2023-09-27" @default.
- W2363090429 title "Specific detection of mycobacterium tuberculosisin clinical material by PCR and southern blot." @default.
- W2363090429 hasPublicationYear "1994" @default.
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