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- W2363094418 abstract "Objective To explore the effect of 20(R)-Ginsenoside Rg3 on lymphatic endothelial cell migration,proliferation and apoptosis.Methods Hela cells were cultured in media containing Rg3,and conditioned media(CM) were prepared with the culture media from hela cells;LECs which ducted from healthy pig were cultured;Ⅷ and VEGFR-3 were used to idenifty LCEs.The methods of craped line and MTT were used to examine the effectiveness of 20(R)-Ginsenoside Rg3 on lymphatic endothelial cell migration and proliferation;Hoechst33258 fluorescence staining was used to detect endothelial cell apoptosis.Results Ⅷ factor and VEGFR-3 antibody were used to identify the cultured lymphatic cells.The result showed that they were typical lymphatic endothelial cells;The scraped line and MTT method were used to compare the difference between the control group and the experimental group.The result showed:different concentrations of 20(R)-Ginsenoside Rg3 can inhibit lymphatic endothelial cell proliferation and migration,the difference was significant(P0.01);Hoechst33258 staining confirmed that apoptotic bodies could be observed around its nuclear in 20(R)-Ginsenoside Rg3 treated LECs.Conclusion 20(R)Ginsenoside Rg3 could evidently inhibit the migration and proliferation of lymphatic endothelial cells and the effects were dosedependent;20(R)-Ginsenoside Rg3 could induce the apoptosis of LECs." @default.
- W2363094418 created "2016-06-24" @default.
- W2363094418 creator A5038301501 @default.
- W2363094418 date "2009-01-01" @default.
- W2363094418 modified "2023-09-23" @default.
- W2363094418 title "Influence of 20(R)-ginsenoside Rg3 on lymphangenesis" @default.
- W2363094418 hasPublicationYear "2009" @default.
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